Journal: The Laryngoscope

Article Title: An in-vitro scaffold-free epithelial-fibroblast coculture model for the larynx

doi: 10.1002/lary.26388

Figure Lengend Snippet: Visualization of experimental design. I-hVFFs were seeded at 3–4 × 104 cells/cm2 and allowed to grow to confluence for 48 hours to enable extracellular matrix formation. After 48 h, ECs were seeded directly on top at a seeding density of 6–7 × 104 cells/cm2, either in (i) complete epithelial media to create a healthy coculture or in (ii) complete epithelial media supplemented with 10 ng/ml TGFβ1 to induce into a fibrotic coculture model

Article Snippet: Primary Bronchial/Tracheal Epithelial Cells (ECs) Primary bronchial/tracheal epithelial cells were purchased from ATCC (Manassas, VA; Catalog # PCS-300-010) and grown in complete airway epithelial cell serum free basal medium (ATCC-PCS-300-030) supplemented with bronchial/tracheal cell growth kit (ATCC-PCS-300-040) and gentamicin/amphotericin B solution (ATCC-PCS-999-025).

Techniques:

Journal: The Laryngoscope

Article Title: An in-vitro scaffold-free epithelial-fibroblast coculture model for the larynx

doi: 10.1002/lary.26388

Figure Lengend Snippet: MTT assay on I-hVFFs for metabolic activity. I-hVFFs performed better in epithelial media at a seeding density of 37,500 cells/cm2 and was hence, chosen for future experiments. As metabolic activity was retained in all medias, epithelial media was chosen as coculture media to ensure that the primary ECs maintain phenotype

Article Snippet: Primary Bronchial/Tracheal Epithelial Cells (ECs) Primary bronchial/tracheal epithelial cells were purchased from ATCC (Manassas, VA; Catalog # PCS-300-010) and grown in complete airway epithelial cell serum free basal medium (ATCC-PCS-300-030) supplemented with bronchial/tracheal cell growth kit (ATCC-PCS-300-040) and gentamicin/amphotericin B solution (ATCC-PCS-999-025).

Techniques: MTT Assay, Activity Assay

Journal: Innate Immunity

Article Title: Susceptibility of primary human airway epithelial cells to Bordetella pertussis adenylate cyclase toxin in two- and three-dimensional culture conditions

doi: 10.1177/1753425920979354

Figure Lengend Snippet: Airway epithelial cells do not express CD11b. Expression of CD11b in airway epithelial cells and THP-1 was analysed by flow cytometry. CD11b-positive cells were not found in HNEC (a), HTEC (b) or HBEC3-KT (c) samples. THP-1 populations were CD11b-positive (d). Blue histograms show isotype control-stained cells and red histograms show cells stained with an anti-CD11b Ab. The figure shows the histograms of one representative experiment ( n =3).

Article Snippet: 14, Airway epithelial cells were grown in Airway Epithelial Cell Growth Medium (AECG; #PB-C-MH-350-0099, PeloBiotech, Martinsried, Germany).

Techniques: Expressing, Flow Cytometry, Control, Staining

Journal: Innate Immunity

Article Title: Susceptibility of primary human airway epithelial cells to Bordetella pertussis adenylate cyclase toxin in two- and three-dimensional culture conditions

doi: 10.1177/1753425920979354

Figure Lengend Snippet: CyaA affects intracellular cAMP concentration and IL-6 secretion in human airway epithelial cells. (a) HNEC, HTEC and HBEC3-KT were incubated with CyaA or CyaA-AC – and intracellular cAMP concentration was analysed ( n = 4). (b) Quantification of human IL-6 secreted by HNEC, HTEC and HBEC3-KT populations after treatment with CyaA or CyaA-AC – was assayed by ELISA of the supernatants ( n = 3). (c) Quantification of human IL-6 secreted by human nasal mucosa tissue models after treatment with CyaA or CyaA-AC – ( n =3). All data are presented as means (bars) + SEM (error bars). Differences after CyaA incubation compared to the control group (TUC) are indicated (* P ≤ 0.05, $ P > 0.05 ≤ 0.1, ANOVA). Statistically significant differences of IL-6 basal levels are indicated ( # P < 0.05, ANOVA). Significant cell type-specific differences are indicated ( & P < 0.05, ANOVA).

Article Snippet: 14, Airway epithelial cells were grown in Airway Epithelial Cell Growth Medium (AECG; #PB-C-MH-350-0099, PeloBiotech, Martinsried, Germany).

Techniques: Concentration Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control